The Effects of Different Doses of Dexmedetomidine on Wound Healing
Erol Karaaslan, Ahmet Selim Özkan, Sedat Akbaş, Yusuf Türköz, Azibe Yıldız, Nuray Üremiş, Emek Güldoğan, Nigar Vardı, Turan Yıldız
Inonu University, Faculty of Medicine, Department of Anesthesiology and Reanimation, Malatya, Turkey
Giriş:
Currently, no existing study shows what the effects of Dexmedetomidine(Dex) are on a surgical wound when it is subcutaneously applied to a surgical wound. Consequently, this study examined the effects of Dex on wound healing.
Gereç ve Yöntem: Rats were randomly divided into 4 groups, comprising 8 rats each: GroupI(Control/3 ml SF), GroupII(5 μg/kg Dex), GroupIII(30 μg/kg Dex), and GroupIV(60 μg/ kg Dex). Study drug mixtures(3 ml) were administered subcutaneously. On the 7th day following the procedure, 5x1 cm samples were taken from the surgical wound line and the rats were sacrificed after 3 ml of blood were drawn(Figure1). The effects of Dex on wound healing were investigated using Arginase, Collagen Type I, Hydroxyproline, Xaa-Pro Dipeptidase/Prolidase, Transforming Growth Factor Beta 1(TGF-β1), and Basic Fibroblast Growth Factor(bFGF), which are the parameters that provide information about collagen synthesis. Collagenization density was evaluated histopathologically semiquantitatively. Immunohistochemically, the immunoreactivity of Proliferating Cell Nuclear Antigen(PCNA) and Vascular Endothelial Growth Factor(VEGF) proteins was examined to determine the proliferation of connective tissue cells and blood vessel development in the dermis.
Bulgular: Hydroxypyrroline (ng/ml) values were higher in Groups II, III, and IV in comparison to Group I and were statistically significant (p < 0.001). When Arginase (ng/ml), Collagen Type I (ng/ml), TGF-β1 (ng/L), and bFGF (ng/L) values were compared, a statistically significant difference was found between the groups (p = 0.043, p = 0.001, p = 0.012, and p = 0.003, respectively)(Table1,Figure2). No statistical difference was found between the groups in relation to Xaa-Pro Dipeptidase/Prolidase (ng/ml). PCNA immunoreactivity was statistically significantly higher in the 30 mcg/kg group in comparison to the other groups (p<0.001). VEGF immunoreactivity was statistically significantly higher in the 5 mcg/kg group (p<0.0001)(Table2,Figure3-4).
Tartışma ve Sonuç: In rats, subcutaneously administered Dex did not have negative effects on wound healing but contributed positively when the analyzed histological and biochemical parameters were evaluated.
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