LOCOMOTOR DIFFERENCES IN MONGOLIAN GERBILS WITH THE EFFECTS OF MIDAZOLAM ADMINISTRATION IN THE FORM OF EYE DROPS
A.AKKAYA
1, UY.TEKELIOGLU
1, A.DEMIRHAN
1, H.BAYIR
1, H.KOCOGLU
1, M.BILGI
1, T.APUHAN
2, M.ERDURMUS
3, E.AYAZ
4
DEPARTMENT OF ANESTHESIOLOGY AND REANIMATION, ABANT IZZET BAYSAL UNIVERSITY MEDICAL SCHOOL, BOLU, TURKEY 1
DEPARTMENT OF HEAD & NECK SURGERY, ABANT IZZET BAYSAL UNIVERSITY MEDICAL SCHOOL, BOLU, TURKEY 2
DEPARTMENT OF OPHTHALMOLOGY, ABANT IZZET BAYSAL UNIVERSITY MEDICAL SCHOOL, BOLU, TURKEY, 3
DEPARTMENT OF PARASITOLOGY, ABANT IZZET BAYSAL UNIVERSITY MEDICAL SCHOOL, BOLU, TURKEY 4
Midazolam isa sedative-hypnotic agent with amnestic and anticonvulsant properties that canbe administrated to mammals through various routes, such as intravenous,intramuscular, oral, intrathecal, rectal, and buccal. Midazolam administrationin the form of eye drops through the conjunctiva is not reported in the literature.
This studyaims to demonstrate the possible central nervous system effects of midazolamadministration as eyes drops in Mongolian gerbils.
Fourteengerbils were randomly assigned to one of two equal sized groups. The activearm received 2 ml of 10 mg midazolam aseye drops in both eyes. Control group received a total of 2 ml of physiologicalsaline(0.9% NaCl). We subjected the gerbils to an adapted “Open Field” todetermine the possible effects on central nervous system of midazolam. Gerbilswere allowed to move freely in the open field. Before and after the drugadministration, locomotor activities of each gerbil have been recorded.Frequency of loss of righting reflex was quantified.
ConjunctivalMidazolam administration resulted with the transient loss of righting reflex(p=0.017) and suppressed exploration motion (p=0.018) in the open field testcompared to control subjects.
In thepresent study, administration of conjunctival midazolam as an eye drop mayaffect gerbil’s locomotor activities and open field behaviors. We argue that,using a sedative and anticonvulsive drug such as midazolam via conjunctivalroute may be useful in some clinical situations. Therefore, it could bebeneficial to develop a new conjunctival formulation of midazolam. Also,thereis a need for trials in humans with pharmacokinetic studies.
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