Effect of Dental Pulp Derived Mesenchymal Stem Cells on Mental Nerve Repair
Zeynep Burçin Gönen1, Dilek Günay Canpolat2, Arzu Yay3, Dilek Bahar4, Nükhet Kütük1, Halis Çolpak1, Betül Yalçın3, Alper Alkan1
1Department of Oral and Maxillofacial Surgery, Erciyes University, Kayseri, Turkey
2Department of Oral and Maxillofacial Surgery, Anesthesiologist, Erciyes University, Kayseri, Turkey
3Department of Histology and Embryology, Medicine Faculty, University of Erciyes, Kayseri
4Department of Stem Cell, Genome and Stem Cell Center, University of Erciyes, Kayseri
OBJECTIVE: Peripheral nerves may damage during the process of peripheral nerve block. An injection inside the nerve or any trauma to the nerve fibers by the needle may cause temporary or permanent damage on the peripheral nerve. The mental nerve is often injured during dentoalveolar, orthognathic, or tumor surgery. Numerous therapeutic interventions, including surgery and pharmacotherapy, have been used to enhance the functional recovery of nerve injuries. However, the clinical effect is usually unsatisfactory, especially after severe injury. The aim of this study was to evaluate the effect of dental pulp derived mesenchymal stem cells (DP-MSCs) for mental nerve regeneration. MATERIALS-METHODS: Human DP-MSCs were isolated from wisdom teeth of healthy donor. Phenotyping was carried out by flow cytometry antibodies specific for CD markers (CD34, CD45, HLA-DR, CD73, and CD90, CD44, and CD105). 10 rabbits were included to study. Mental nerve of rabbits were transected in order to mimic a injury bilaterally. A microsurgical repair was then immediately achieved. In control group sterile saline were applied to the area however 2 x 10 6 DP-MSCs were applied in study group. After sacrification, for evaluating to quantitative histomorphometry of carefully defined reference areas counted the number of total axons with Image J software program. RESULTS: At the end of passage 3, phenotype characterization of the DP-MSCs demonstrated a homogeneous population of cells negative for CD34, CD45, HLA-DR and positive for CD44, CD73, CD90, and CD105. Microscopic examination of the nerve showed degenerated axons and axonal connective tissue loosing between axons in control group. According to histomorphometric analyses, our finding clearly demonstrated that lower number of cross-sectioned axons were founded in control group compaired to study group. But there was also less fibers in control. CONCLUSION: DP-MSCs may have positive effect of mental nerve regeneration.
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